BIOCHEMISTRY- MEDICAL DEPARTMENT

BIOCHEMISTRY-MEDICAL DEPARTMENT

BIOCHEMISTRY- MEDICAL DEPARTMENT SYLLABUS

(These syllabus may change from time to time. Please check with the relevant Public Service Commission websites for any changes in the syllabus)

1. Basic Laboratory Procedures:  Disinfection, Laboratory safety, first aid.  Biochemical samples and their collection, preservatives, anticoagulants.  Preparation of solutions and reagents.  Laboratory reporting systems, biological variations, chemical interference, Normal/reference range.  Expression of results of biochemical investigations-various units.  Quality management of laboratory- standardization and calibration.  Biomedical waste disposal.  Laboratory record keeping, Accreditation.
2. Biochemical Instruments/Technique: Colorimetry, Spectrophotometry, Fluorometry, Chromatography, Electrophoresis, Densitometer, Flame photometry, Electrolyte analyser, Blood gas analyser, Atomic absorption spectroscopy, RIA, ELISA, Gamma counter, Chemiluminescence assay, Water purification systems, Semi autoanalyser, Fully autoanalyser, PCR, HPLC, Blotting technique.
3. Estimation of various biochemical parameters.
4. Organ function tests: Liver, kidney, thyroid, pancreas, adrenocortical.
5. Chemistry, metabolism & Inborn errors of: (a) Carbohydrates (b) Lipids (c) Amino Acid & Proteins (d) Nucleotides
6. Enzymes
7. Vitamins
8. Mineral Metabolism and abnormalities
9. Acid-base balance & pH
10. Electrolyte and water balance.
11. Heme synthesis & Breakdown
12. Energy metabolism & Nutrition
13. Hormones
14. Environmental Biochemistry
15. Immunochemistry
16. Biochemistry of Cancer
17. Biochemistry of HIV & AIDS

BIOCHEMISTRY MCQs

Ques # :1
Which of the following is not a single membrane bound cell organelles
1) Golgi bodies
2) Ribosome
3) Peroxisomes
4) Lysosomes
Ans: 2

Ques # :2
Which of the following has the lowest glycemic index
1) Maltose
2) Fructose
3) Milk
4) Polished rice
Ans: 2

Ques # :3
The primary transporter for fructose in small intestine and testes:
1) GLUT-1
2) GLUT-5
3) GLUT-3
4) GLUT-2
Ans: 2

Ques # :4
The patients excrete milky urine in disorder
1) Ketonuria
2) Chyluria
3) Lactosuria
4) Galactosuria
Ans: 2

Ques # :5
Lysolecithin is formed from lecithin by the action of
1) Phospholipase A1
2) Phospholipase A2
3) Phospholipase C
4) Phospholipase D
Ans: 2

Ques # :6
Which of the following is considered as a dignostic marker for early apoptotis
1) Cardiolipin
2) Gangliosides
3) Cephalin
4) Aryl Sulphate
Ans: 1

Ques # :7
Allosteric inhibitor of gultamate dehydrogenase enzyme is
1) AMP
2) ATP
3) GMP
4) ADP
Ans: 2

Ques # :8
Ubiquitin is required for
1) Lysosomal degradation of proteins
2) Synthesis of ubiquinone
3) Cytosolic degradation of proteins
4) Synthesis of urea
Ans: 3

Ques # :9
The commited step in biosynthesis of pyrimidine is catalysed by
1) dihydrorotase
2) dihydrorotase dehydrogenase
3) Carbamoylphosphate synthetase
4) Aspartate transcarbamoylase
Ans: 4

Ques # :10
Which of the following has the strongest tendency to gain electrons
1) Co Enzyme Q
2) Cytochrome C
3) NAD
4) Oxygen
Ans: 4