BIOCHEMISTRY- PAGE 1
BIOCHEMISTRY- MEDICAL DEPARTMENT MCQs
1:-Alfred Hershey and Martha Chase during their experiment on phase DNA used following radioisotopes for labeling
A:-`"^3H"` and `"^{15}N"`
B:-`"^{14}C"` and `"^{131}I"`
C:-`"^{32}P"` and `"^{35}S"`
D:-`"^3H"` and `"^{60}Co"`
Ans: C
2:-Pentaglycine chain bridges the lysine E-amino group and the D-alanine carboxylic group in the cell wall of
A:-Gram positive bacteria
B:-Gram negative bacteria
C:-Fungi
D:-Higher plants
Ans: A
3:-Lactate to pyruvate conversion takes place in
A:-Muscle
B:-Liver
C:-Muscle and liver
D:-None of these
Ans: B
4:-Attenuational control of gene expression occurs in
A:-Bacteria
B:-Mitochondria
C:-Chloroplasts
D:-All of the above
Ans: D
5:-Which of the following RNA types is comparatively least stable in bacteria?
A:-mRNA
B:-tRNA
C:-rRNA
D:-All are equally stable
Ans: A
6:-The proteins that redistribute lipids across the membrane belong to the class
A:-Flippases
B:-Floppases
C:-Scramblasses
D:-All of the above
Ans: D
7:-Selenocysteine is coded by
A:-AUG
B:-UAA
C:-UAG
D:-UGA
Ans: D
8:-Predict the order of emergence of the following proteins when a mixture of four is chromatographed on a gel exclusion column at pH 7
Protein 1 ; Mr 6,000 pI = 5
Protein 2 ; Mr 23,000 pI = 8
Protein 3 ; Mr 55,000 pI = 4
Protein 4 ; Mr 10,000 pI = 10
A:-Protein 1, Protein 2, Protein 3, Protein 4
B:-Protein 3, Protein 2, Protein 4, Protein 1
C:-Protein 4, Protein 2, Protein 1, Protein 3
D:-Protein 3, Protein 1, Protein 2, Protein 4
Ans: B
9:-Which of the following is anion exchange resin?
A:-CM cellulose
B:-Dowex-50
C:-DEAE cellulose
D:-CM sepharose
Ans: C
10:-How many bands will appear when hemoglobin is electrophoresed on SDS PAGE in reducing condition?
A:-1
B:-2
C:-3
D:-4
Ans: B
11:-Major emission band during measurement of sodium concentration by flame emission spectroscopy is obtained at the wave length
A:-589 nm
B:-671 nm
C:-786 nm
D:-852 nm
Ans: A
12:-Identify the commonly used mobile phase in GC-MS.
A:-`"CH_3CN"`
B:-`"H_2O"`
C:-He
D:-`"CO_2"`
Ans: C
13:-For studying protein dynamics in live cells fluorescence recovery after photobleaching (FRAP) is used. It is based on
A:-Rapid image acquisition of live cells
B:-Mobility of particular fusion protein
C:-Reversible photobleaching of fluorescent protein
D:-Irreversible photobleaching of fluorescent protein
Ans: B
14:-Which combination of ligand and target molecule is wrongly written about affinity chromatography?
A:-Antibody-antigen
B:-Hormone-receptor
C:-Avidin-biotin
D:-Lectin-immunoglobulin
Ans: D
15:-Which of the following dye is used for staining denatured linear RNA during electrophoresis?
A:-Ethydium bromide
B:-Bromophenol blue
C:-Commassie brilliant blue
D:-Acridine orange
Ans: D
16:-Fundamental vibrational motions are studied in
A:-IR spectroscopy
B:-UV-Vis spectroscopy
C:-Circular dichroism
D:-Mass spectrometry
Ans: A
17:-Diapedesis is
A:-An increase in diameter of blood vessels
B:-An increase in capillary permeability
C:-Emigration of phagocytes through the gap between endothelial cells to inflammation site
D:-Adherence of cells to the endothelial wall
Ans: C
18:-Half life of `"^{14}C"` is about 5760 years. After how many years will you get about 3% of the original activity?
A:-5760 years
B:-17280 years
C:-28800 years
D:-34560 years
Ans: C
19:-The separation of plasma proteins by electrophoresis is commonly carried out at pH
A:-10.0
B:-8.6
C:-7.4
D:-6.5
Ans: B
20:-Which of the following does not participate in antioxidant defense system?
A:-SOD
B:-Catalase
C:-Glutathione
D:-Glucokinase
Ans: D
21:-The enzyme present in Indian cobra venom, responsible for mediating toxic effect, is
A:-Proteinases
B:-Nucleases
C:-Phospholipase
D:-Carbohydrases
Ans: C
22:-Telomerase
A:-is a ribonucleoprotein
B:-is a RNA dependent DNA polymerase
C:-uses 3' end of DNA as primer and adds successive TTAGGG repeats to it
D:-all of the above
Ans: D
23:-Diisopropylphosphofluoridate is an irreversible inhibitor of the enzyme
A:-Acetylcholinesterase
B:-Glycopeptide transpeptidase
C:-Cytochrome oxidase
D:-Succinate dehydrogenase
Ans: A
24:-Reverse transcriptase has
A:-RNA dependent DNA polymerase activity
B:-DNA dependent DNA polymerase activity
C:-RNase H activity
D:-All of the above
Ans: D
25:-Which of the following is lacking in muscle, but present in normal liver?
A:-Glycogen phosphorylase
B:-Glucose-6-phosphatase
C:-Phosphoglucomutase
D:-Phosphoglucokinase
Ans: B
26:-Full form of DPT vaccine is
A:-Diphtheria, Pertusis, Tetanus
B:-Diphtheria, Pertusis, Typhoid
C:-Diphtheria, Pneumonia, Tetanus
D:-Dysentry, Polio, Tetanus
Ans: A
27:-Penicillin inhibits bacterial growth by irreversibly inactivating the enzyme
A:-Fatty acid synthase
B:-Glycopeptide transpeptidase
C:-Cytochrome oxidase
D:-Hexokinase
Ans: B
28:-Which statistical test is used for comparison of two means from independent samples?
A:-F-test
B:-t-test
C:-Chi-square test
D:-Z-test
Ans: B
29:-Which of the following acts like detergent?
A:-Isoprene
B:-Phospholipids
C:-Deoxycholic acid
D:-Estradiols
Ans: C
30:-Splicing of GU-AG intron
A:-Involves transesterification reaction
B:-Form Y-shaped branch structure
C:-Occurs in prokaryotic messenger RNA
D:-Requires maturase activity
Ans: A
A:-`"^3H"` and `"^{15}N"`
B:-`"^{14}C"` and `"^{131}I"`
C:-`"^{32}P"` and `"^{35}S"`
D:-`"^3H"` and `"^{60}Co"`
Ans: C
2:-Pentaglycine chain bridges the lysine E-amino group and the D-alanine carboxylic group in the cell wall of
A:-Gram positive bacteria
B:-Gram negative bacteria
C:-Fungi
D:-Higher plants
Ans: A
3:-Lactate to pyruvate conversion takes place in
A:-Muscle
B:-Liver
C:-Muscle and liver
D:-None of these
Ans: B
4:-Attenuational control of gene expression occurs in
A:-Bacteria
B:-Mitochondria
C:-Chloroplasts
D:-All of the above
Ans: D
5:-Which of the following RNA types is comparatively least stable in bacteria?
A:-mRNA
B:-tRNA
C:-rRNA
D:-All are equally stable
Ans: A
6:-The proteins that redistribute lipids across the membrane belong to the class
A:-Flippases
B:-Floppases
C:-Scramblasses
D:-All of the above
Ans: D
7:-Selenocysteine is coded by
A:-AUG
B:-UAA
C:-UAG
D:-UGA
Ans: D
8:-Predict the order of emergence of the following proteins when a mixture of four is chromatographed on a gel exclusion column at pH 7
Protein 1 ; Mr 6,000 pI = 5
Protein 2 ; Mr 23,000 pI = 8
Protein 3 ; Mr 55,000 pI = 4
Protein 4 ; Mr 10,000 pI = 10
A:-Protein 1, Protein 2, Protein 3, Protein 4
B:-Protein 3, Protein 2, Protein 4, Protein 1
C:-Protein 4, Protein 2, Protein 1, Protein 3
D:-Protein 3, Protein 1, Protein 2, Protein 4
Ans: B
9:-Which of the following is anion exchange resin?
A:-CM cellulose
B:-Dowex-50
C:-DEAE cellulose
D:-CM sepharose
Ans: C
10:-How many bands will appear when hemoglobin is electrophoresed on SDS PAGE in reducing condition?
A:-1
B:-2
C:-3
D:-4
Ans: B
11:-Major emission band during measurement of sodium concentration by flame emission spectroscopy is obtained at the wave length
A:-589 nm
B:-671 nm
C:-786 nm
D:-852 nm
Ans: A
12:-Identify the commonly used mobile phase in GC-MS.
A:-`"CH_3CN"`
B:-`"H_2O"`
C:-He
D:-`"CO_2"`
Ans: C
13:-For studying protein dynamics in live cells fluorescence recovery after photobleaching (FRAP) is used. It is based on
A:-Rapid image acquisition of live cells
B:-Mobility of particular fusion protein
C:-Reversible photobleaching of fluorescent protein
D:-Irreversible photobleaching of fluorescent protein
Ans: B
14:-Which combination of ligand and target molecule is wrongly written about affinity chromatography?
A:-Antibody-antigen
B:-Hormone-receptor
C:-Avidin-biotin
D:-Lectin-immunoglobulin
Ans: D
15:-Which of the following dye is used for staining denatured linear RNA during electrophoresis?
A:-Ethydium bromide
B:-Bromophenol blue
C:-Commassie brilliant blue
D:-Acridine orange
Ans: D
16:-Fundamental vibrational motions are studied in
A:-IR spectroscopy
B:-UV-Vis spectroscopy
C:-Circular dichroism
D:-Mass spectrometry
Ans: A
17:-Diapedesis is
A:-An increase in diameter of blood vessels
B:-An increase in capillary permeability
C:-Emigration of phagocytes through the gap between endothelial cells to inflammation site
D:-Adherence of cells to the endothelial wall
Ans: C
18:-Half life of `"^{14}C"` is about 5760 years. After how many years will you get about 3% of the original activity?
A:-5760 years
B:-17280 years
C:-28800 years
D:-34560 years
Ans: C
19:-The separation of plasma proteins by electrophoresis is commonly carried out at pH
A:-10.0
B:-8.6
C:-7.4
D:-6.5
Ans: B
20:-Which of the following does not participate in antioxidant defense system?
A:-SOD
B:-Catalase
C:-Glutathione
D:-Glucokinase
Ans: D
21:-The enzyme present in Indian cobra venom, responsible for mediating toxic effect, is
A:-Proteinases
B:-Nucleases
C:-Phospholipase
D:-Carbohydrases
Ans: C
22:-Telomerase
A:-is a ribonucleoprotein
B:-is a RNA dependent DNA polymerase
C:-uses 3' end of DNA as primer and adds successive TTAGGG repeats to it
D:-all of the above
Ans: D
23:-Diisopropylphosphofluoridate is an irreversible inhibitor of the enzyme
A:-Acetylcholinesterase
B:-Glycopeptide transpeptidase
C:-Cytochrome oxidase
D:-Succinate dehydrogenase
Ans: A
24:-Reverse transcriptase has
A:-RNA dependent DNA polymerase activity
B:-DNA dependent DNA polymerase activity
C:-RNase H activity
D:-All of the above
Ans: D
25:-Which of the following is lacking in muscle, but present in normal liver?
A:-Glycogen phosphorylase
B:-Glucose-6-phosphatase
C:-Phosphoglucomutase
D:-Phosphoglucokinase
Ans: B
26:-Full form of DPT vaccine is
A:-Diphtheria, Pertusis, Tetanus
B:-Diphtheria, Pertusis, Typhoid
C:-Diphtheria, Pneumonia, Tetanus
D:-Dysentry, Polio, Tetanus
Ans: A
27:-Penicillin inhibits bacterial growth by irreversibly inactivating the enzyme
A:-Fatty acid synthase
B:-Glycopeptide transpeptidase
C:-Cytochrome oxidase
D:-Hexokinase
Ans: B
28:-Which statistical test is used for comparison of two means from independent samples?
A:-F-test
B:-t-test
C:-Chi-square test
D:-Z-test
Ans: B
29:-Which of the following acts like detergent?
A:-Isoprene
B:-Phospholipids
C:-Deoxycholic acid
D:-Estradiols
Ans: C
30:-Splicing of GU-AG intron
A:-Involves transesterification reaction
B:-Form Y-shaped branch structure
C:-Occurs in prokaryotic messenger RNA
D:-Requires maturase activity
Ans: A
31:-Fatty acids occurring in humans most commonly
A:-are highly branched structure
B:-have double bonds present in trans configuration
C:-contain an even number of carbon atoms
D:-are limited to 16 or fewer carbon atoms
Ans: C
32:-The active site of all serine proteases contain
A:-Histidine
B:-Asparagine
C:-`gamma`-carboxyglutamate
D:-Lysine or arginine
Ans: A
33:-If a polypeptide has 400 amino acid residues, what is its approximate mass?
A:-22,000 daltons
B:-40,000 daltons
C:-44,000 daltons
D:-80,000 daltons
Ans: C
34:-Gamma(`gamma`)-carboxyglutamate, a derived amino acid is found in
A:-elastin
B:-collagen
C:-hemoglobin
D:-prothrombin
Ans: D
35:-Which of the following consensus sequence is present at -35 position of a typical bacterial promoter?
A:-TTGACA
B:-TATAAT
C:-GAATTC
D:-None of the above
Ans: A
36:-Which of the following contains a hexahydroxy alcohol?
A:-Gangliosides
B:-Phosphatidylcholines
C:-Phosphatidylinositols
D:-Sphingomyelins
Ans: C
37:-The amide nitrogen of glutamine
A:-represents a nontoxic transport form of ammonia
B:-is used in synthesis of asparagine, purines and pyrimidines
C:-can be recovered as ammonia by the action of glutaminase
D:-all of the above
Ans: D
38:-Artificial sweeteners sugar free and equal contain aspartame. Name the two amino acids that are components of aspartame
A:-Aspartic acid and phenylalanine
B:-Arginine and tyrosine
C:-Aspartic acid and tryptophan
D:-Aspartic acid and glutamic acid
Ans: A
39:-Amino acids present in glutathione include
A:-Glutamate, cysteine, glycine
B:-Aspartate, cysteine, glycine
C:-Glutamate, cysteine, lysine
D:-Glutamate, methionine, glycine
Ans: A
40:-Molar concentration of `"H_2O"` in pure water is
A:-30.5 M
B:-55.5 M
C:-75 M
D:-100 M
Ans: B
41:-Lactate dehydrogenase reaction is monitored by formation of NADH at wavelength
A:-260
B:-280
C:-340
D:-420
Ans: C
42:-Hyaluronate, an important component of vitreous humor and synovial fluid, is
A:-A sugar alcohol
B:-A glycosaminoglycan
C:-A glycoprotein
D:-A glycol lipid
Ans: B
43:-Statins are routinely used for treatment of hypercholesterolemia. They mediate their action by inhibiting
A:-AMP-activated protein kinase
B:-Lecithin-cholesterol acyl transferase
C:-Prostaglandin synthase
D:-Hydroxyl methyl glutaryl CoA reductase
Ans: D
44:-In presence of competitive inhibitor in enzyme catalysed reactions
A:-Vmax increases
B:-Vmax decreases
C:-Km increases
D:-Km decreases
Ans: C
45:-A peptide has a sequence Glu-His-Trp-Ser-Gly-Leu-Lys-Pro-Gly. What is the net charge on the molecule at pH 11?
A:-+2
B:--1
C:-0
D:--2
Ans: D
46:-The alpha carboxyl group of all the 20 standard amino acids have pK value in the range
A:-1.8 - 2.9
B:-4.2 - 6.5
C:-7.2 - 8.2
D:-8.8 - 10.8
Ans: A
47:-IL-2 secretion induces
A:-T-cell proliferation
B:-Vasculature formation
C:-Antiviral activity
D:-MHC-1 expression
Ans: A
48:-Which of the following is used as homology and similarity tool?
A:-EMBOSS
B:-RAMSOL
C:-BLAST
D:-PROSPECT
Ans: C
49:-Abzymes are
A:-Proenzymes
B:-Enzymes that hydrolyse antibiotics
C:-Enzymes present in biodetergents
D:-Antibodies having catalytic activities
Ans: D
A:-are highly branched structure
B:-have double bonds present in trans configuration
C:-contain an even number of carbon atoms
D:-are limited to 16 or fewer carbon atoms
Ans: C
32:-The active site of all serine proteases contain
A:-Histidine
B:-Asparagine
C:-`gamma`-carboxyglutamate
D:-Lysine or arginine
Ans: A
33:-If a polypeptide has 400 amino acid residues, what is its approximate mass?
A:-22,000 daltons
B:-40,000 daltons
C:-44,000 daltons
D:-80,000 daltons
Ans: C
34:-Gamma(`gamma`)-carboxyglutamate, a derived amino acid is found in
A:-elastin
B:-collagen
C:-hemoglobin
D:-prothrombin
Ans: D
35:-Which of the following consensus sequence is present at -35 position of a typical bacterial promoter?
A:-TTGACA
B:-TATAAT
C:-GAATTC
D:-None of the above
Ans: A
36:-Which of the following contains a hexahydroxy alcohol?
A:-Gangliosides
B:-Phosphatidylcholines
C:-Phosphatidylinositols
D:-Sphingomyelins
Ans: C
37:-The amide nitrogen of glutamine
A:-represents a nontoxic transport form of ammonia
B:-is used in synthesis of asparagine, purines and pyrimidines
C:-can be recovered as ammonia by the action of glutaminase
D:-all of the above
Ans: D
38:-Artificial sweeteners sugar free and equal contain aspartame. Name the two amino acids that are components of aspartame
A:-Aspartic acid and phenylalanine
B:-Arginine and tyrosine
C:-Aspartic acid and tryptophan
D:-Aspartic acid and glutamic acid
Ans: A
39:-Amino acids present in glutathione include
A:-Glutamate, cysteine, glycine
B:-Aspartate, cysteine, glycine
C:-Glutamate, cysteine, lysine
D:-Glutamate, methionine, glycine
Ans: A
40:-Molar concentration of `"H_2O"` in pure water is
A:-30.5 M
B:-55.5 M
C:-75 M
D:-100 M
Ans: B
41:-Lactate dehydrogenase reaction is monitored by formation of NADH at wavelength
A:-260
B:-280
C:-340
D:-420
Ans: C
42:-Hyaluronate, an important component of vitreous humor and synovial fluid, is
A:-A sugar alcohol
B:-A glycosaminoglycan
C:-A glycoprotein
D:-A glycol lipid
Ans: B
43:-Statins are routinely used for treatment of hypercholesterolemia. They mediate their action by inhibiting
A:-AMP-activated protein kinase
B:-Lecithin-cholesterol acyl transferase
C:-Prostaglandin synthase
D:-Hydroxyl methyl glutaryl CoA reductase
Ans: D
44:-In presence of competitive inhibitor in enzyme catalysed reactions
A:-Vmax increases
B:-Vmax decreases
C:-Km increases
D:-Km decreases
Ans: C
45:-A peptide has a sequence Glu-His-Trp-Ser-Gly-Leu-Lys-Pro-Gly. What is the net charge on the molecule at pH 11?
A:-+2
B:--1
C:-0
D:--2
Ans: D
46:-The alpha carboxyl group of all the 20 standard amino acids have pK value in the range
A:-1.8 - 2.9
B:-4.2 - 6.5
C:-7.2 - 8.2
D:-8.8 - 10.8
Ans: A
47:-IL-2 secretion induces
A:-T-cell proliferation
B:-Vasculature formation
C:-Antiviral activity
D:-MHC-1 expression
Ans: A
48:-Which of the following is used as homology and similarity tool?
A:-EMBOSS
B:-RAMSOL
C:-BLAST
D:-PROSPECT
Ans: C
49:-Abzymes are
A:-Proenzymes
B:-Enzymes that hydrolyse antibiotics
C:-Enzymes present in biodetergents
D:-Antibodies having catalytic activities
Ans: D
50:-Triple stranded collagen helix is stabilized by
A:-Leucine and isoleucine
B:-Proline and hydroxyproline
C:-Phenylalanine and valine
D:-Methionine and cysteine
Ans: B
51:-Which of the following is a DNA binding motif?
A:-helix-loop-helix
B:-leucine zipper
C:-zinc finger
D:-all of the above
Ans: D
52:-The ideal instrument used for getting accurate mass data in peptide mass finger printing is
A:-Electrophoretic apparatus
B:-MALDI-TOF MS instrument
C:-Spectrophotometer
D:-Ultracentrifuge
Ans: B
53:-Which of the following is tumor suppressor gene?
A:-WT1
B:-Src
C:-Myc
D:-None of these
Ans: A
54:-Which organelle is involved in the oxidation of very long-chain fatty acids?
A:-Peroxisomes
B:-Nucleus
C:-Endoplasmic reticulum
D:-Golgi apparatus
Ans: A
55:-Hyper secretion of ACTH is associated with
A:-Amenorrhoea
B:-Cushing's disease
C:-Steatorrea
D:-Zollinger-Ellison syndrome
Ans: B
56:-Visualization of distribution of transmembrane protein in the plane of a cell membrane can be done in best way by using
A:-Phase contrast microscope
B:-Freeze fracture electron microscopy
C:-Scanning electron microscopy
D:-Affinity chromatography
Ans: B
57:-Ziehl Neelsen staining procedure is applied for staining
A:-Mycobacterium
B:-Shigella spp.
C:-E. coli
D:-Candida spp.
Ans: A
58:-Which of the following is not a gluconeogenic enzyme?
A:-Phosphoenolpyruvate carboxykinase
B:-Fructose 1,6-bisphosphatase
C:-Glucose 6-phosphatase
D:-Phosphoglucomutase
Ans: D
59:-Which of the following antitumor agents works by impairing de novo purine synthesis?
A:-Acyclovir
B:-5-Fluorouracil
C:-Methotrexate
D:-Hydroxyurea
Ans: C
60:-Which of the following is a method for assessing genome complexity?
A:-Annealing
B:-Electrophoresis
C:-Equilibrium centrifugation
D:- `"C_0t"` curves
Ans: D
61:-The direct effect of cAMP in the protein kinase A pathway is to
A:-activate adenylate cyclase
B:-dissociate regulatory subunits from protein kinase
C:-phosphorylate certain cellular proteins
D:-phosphorylate proteins kinase A
Ans: B
62:-Gamma glutamyl transferase is commonly used marker for diagnosis of
A:-Cardiac diseases
B:-Diabetes
C:-Alzheimer disease
D:-Liver diseases
Ans: D
63:-The histamine is responsible for causing
A:-Vasodilation
B:-Increased capillary permeability
C:-Broncho constriction
D:-All of the above
Ans: D
64:-Which of the following pairs of vectors and DNA insert sizes is correctly matched?
A:-Plasmids 5-10 kb
B:-Cosmids 15 kb
C:-YACs 5000 kb
D:-Bacteriophage lambda 45 kb
Ans: A
65:-How many high-energy bonds are used in the formation of one peptide bond?
A:-1
B:-2
C:-3
D:-4
Ans: D
66:-DNA polymerase III lacks following activities
A:-5 - 3' polymerase
B:-3 - 5' exonuclease
C:-5 - 3' exonuclease
D:-All of the above
Ans: C
67:-Triskelion structure is associated with
A:-Glycophorin A
B:-Clathrin
C:-Elastases
D:-Nucleosomes
Ans: B
A:-Leucine and isoleucine
B:-Proline and hydroxyproline
C:-Phenylalanine and valine
D:-Methionine and cysteine
Ans: B
51:-Which of the following is a DNA binding motif?
A:-helix-loop-helix
B:-leucine zipper
C:-zinc finger
D:-all of the above
Ans: D
52:-The ideal instrument used for getting accurate mass data in peptide mass finger printing is
A:-Electrophoretic apparatus
B:-MALDI-TOF MS instrument
C:-Spectrophotometer
D:-Ultracentrifuge
Ans: B
53:-Which of the following is tumor suppressor gene?
A:-WT1
B:-Src
C:-Myc
D:-None of these
Ans: A
54:-Which organelle is involved in the oxidation of very long-chain fatty acids?
A:-Peroxisomes
B:-Nucleus
C:-Endoplasmic reticulum
D:-Golgi apparatus
Ans: A
55:-Hyper secretion of ACTH is associated with
A:-Amenorrhoea
B:-Cushing's disease
C:-Steatorrea
D:-Zollinger-Ellison syndrome
Ans: B
56:-Visualization of distribution of transmembrane protein in the plane of a cell membrane can be done in best way by using
A:-Phase contrast microscope
B:-Freeze fracture electron microscopy
C:-Scanning electron microscopy
D:-Affinity chromatography
Ans: B
57:-Ziehl Neelsen staining procedure is applied for staining
A:-Mycobacterium
B:-Shigella spp.
C:-E. coli
D:-Candida spp.
Ans: A
58:-Which of the following is not a gluconeogenic enzyme?
A:-Phosphoenolpyruvate carboxykinase
B:-Fructose 1,6-bisphosphatase
C:-Glucose 6-phosphatase
D:-Phosphoglucomutase
Ans: D
59:-Which of the following antitumor agents works by impairing de novo purine synthesis?
A:-Acyclovir
B:-5-Fluorouracil
C:-Methotrexate
D:-Hydroxyurea
Ans: C
60:-Which of the following is a method for assessing genome complexity?
A:-Annealing
B:-Electrophoresis
C:-Equilibrium centrifugation
D:- `"C_0t"` curves
Ans: D
61:-The direct effect of cAMP in the protein kinase A pathway is to
A:-activate adenylate cyclase
B:-dissociate regulatory subunits from protein kinase
C:-phosphorylate certain cellular proteins
D:-phosphorylate proteins kinase A
Ans: B
62:-Gamma glutamyl transferase is commonly used marker for diagnosis of
A:-Cardiac diseases
B:-Diabetes
C:-Alzheimer disease
D:-Liver diseases
Ans: D
63:-The histamine is responsible for causing
A:-Vasodilation
B:-Increased capillary permeability
C:-Broncho constriction
D:-All of the above
Ans: D
64:-Which of the following pairs of vectors and DNA insert sizes is correctly matched?
A:-Plasmids 5-10 kb
B:-Cosmids 15 kb
C:-YACs 5000 kb
D:-Bacteriophage lambda 45 kb
Ans: A
65:-How many high-energy bonds are used in the formation of one peptide bond?
A:-1
B:-2
C:-3
D:-4
Ans: D
66:-DNA polymerase III lacks following activities
A:-5 - 3' polymerase
B:-3 - 5' exonuclease
C:-5 - 3' exonuclease
D:-All of the above
Ans: C
67:-Triskelion structure is associated with
A:-Glycophorin A
B:-Clathrin
C:-Elastases
D:-Nucleosomes
Ans: B
68. Transfer of an amino group from an amino acid to an alpha beto acid is done by:
1) Transaminases 2) Aminases 3) Transketolase 4) Decarboxylase
Ans: 1
69. Which product of citric acid cycle is used in detoxification of ammonia in brain
1) Oxaloacetate 2) Alpha ketoglutarate 3) Succinate 4) Citrate
Ans: 2
70. Protein act as buffer due to which property
1) Colloid 2) Basis 3) Acidic 4) Amphipathic
Ans: 4
71.Ammonia is detoxified in brain to:
1) Urea 2) Glutamine 3) GABA 4) Uric acid
Ans: 2
72. Urea cycle is present in:
1) Liver 2) GIT 3) Spleen 4) Kidney
Ans: 1
73. Protein is purified using ammonium sulfate by:
1) Salting out 2) Ion exchange chromatography
3) Mass chromatography 4) Molecular size exclusion
Ans: 1
74. Aromatic ring is presented in
1) Arginine 2) Glycine 3) Phenylalanine 4) Lysine
Ans: 3
75. Protein that precipitate on heating to 45° C & redissolve on boiling is:
1) Bence Jones Protein 2) gamma globulin 3) Albumin 4) Myosin
Ans: 1
76. Tyrosinase is:
1) Oxidase 2) Transferase 3) Lyase 4) Isomerase
Ans: 1
77. Mitochondrial DNA is
1) Paternally inherited 2) Maternally inherited 3) Horizontal inheritance 4) Vertical inheritance
Ans: 2
78. Which amino acid migrates fastest on Chromatography on carboxymethyl cellulose medium
1) Aspartic acid 2) Valine 3) Lysin 4) Glycine
Ans: 1
79. Non functional plasma enzymes are all except
1) Alkaline Phosphotase 2) Acid Phophotase 3) Lipoprotein lipase 4) gamma glutamyl transpeptidase
Ans: 3
80. End of chromosomes are replicated by:
1) Telomerase 2) Centromese 3) Restriction Endonuclease 4) Exonuclease
Ans: 1
81. Okazaki segments are required for:
1) DNA synthesis 2) RNA synthesis
3) Protei synthesis 4) None of them
Ans: 1
82. Refsum's disease is due to deficiency of which of the following enzyme
1) Malonate dehydrogenase 2) Thiophorase 3) Succinate thiopase 4) Phytanic acid oxidase
Ans: 4
83. Which process separates the fragments of DNA
1) Gel Centrifugation 2) Paper chromatography
3) High speed centrifugation 4) Thin layer Chromatography
Ans: 1
84. Sulphur containing amino acid is
1) Asparagine 2) Methionine 3) Glycine 4) Alanine
Ans: 2
85. The main function of mitochondria is
1) Protein synthesis 2) Oxidation 3) Electron transfer 4) Fat synthesis
Ans: 2
86. Increased risk of MI is associated with which amino acid
1) Methionine 2) Hymocysteine 3) Ornithine 4) Valine
Ans: 2
87. UV light damage to the DNA leads to
1) Formation of pyrimydine dimers
2) No damage to DNA
3) DNA hydrolysis
4) Double stranded breaks
Ans: 1
88. The tertiary structure of Protein is detected by:
1) X-ray diffraction/Crystallography
2) Spectrophotometry
3) Electrophoresis
4) Chromatography
Ans: 1
89. Vitamin A is stored mainly as Retinolesters in
1) Kidneys 2) Muscle 3) Liver 4) Retina
Ans: 3
90. Enzymes not involved in glycolysis is
1) Enolase
2) Phosphoglyceromutase
3) aldolase
4) Glycerophosphate dehydrogenase
Ans: 4
91. Which of the following has no free aldehyde or ketone group
1) Fructose 2) Maltose 3) Sucrose 4) Galactose
Ans: 3
92. Part of mRNA removed during protein synthesis
1) Intron 2) Codon
3) Exon 4) Cistron
Ans: 1
93. The sugar component of cerebrosides is
1) Fructose 2) Sucrose 3) Galactose 4) Maltose
Ans: 3
94. Mousy odour urine is seen in
1) Maple syrup urine disease 2) PKU 3) Isovaleric aciduria 4) Cystinuria
Ans: 2
95. Pyruvate dehydrogenase contains all except
1) Biotin 2) NAD 3) FAD 4) CoA
Ans: 1
96. Protein are sorted by:
1) Golgi bodies 2) Mitochondria 3) Ribosomes 4) Nuclear Membrane
Ans: 1
97. Muscles are not involved in which glycogen storage disease
1) I
2) II
3) III
4) IV
Ans: 1
98. The activity of carboxylase is dependent upon the positive allosteric effect of:
1) Succinate
2) AMP
3) Isocitrate
4) Acetyl CoA
Ans: 4
99. Ribosomes has following enzymatic activity
1) peptidyl tranferase 2) Peptidase
3) Aminoacycle + RNA synthelase 4) Gtpase
Ans: 1
100. The substance essential for transfer of fatty acids across mitochondrial membrane
1) Creatine 2) Creatinin 3) Carnitine 4) Coenzyme A
Ans: 3
1) Transaminases 2) Aminases 3) Transketolase 4) Decarboxylase
Ans: 1
69. Which product of citric acid cycle is used in detoxification of ammonia in brain
1) Oxaloacetate 2) Alpha ketoglutarate 3) Succinate 4) Citrate
Ans: 2
70. Protein act as buffer due to which property
1) Colloid 2) Basis 3) Acidic 4) Amphipathic
Ans: 4
71.Ammonia is detoxified in brain to:
1) Urea 2) Glutamine 3) GABA 4) Uric acid
Ans: 2
72. Urea cycle is present in:
1) Liver 2) GIT 3) Spleen 4) Kidney
Ans: 1
73. Protein is purified using ammonium sulfate by:
1) Salting out 2) Ion exchange chromatography
3) Mass chromatography 4) Molecular size exclusion
Ans: 1
74. Aromatic ring is presented in
1) Arginine 2) Glycine 3) Phenylalanine 4) Lysine
Ans: 3
75. Protein that precipitate on heating to 45° C & redissolve on boiling is:
1) Bence Jones Protein 2) gamma globulin 3) Albumin 4) Myosin
Ans: 1
76. Tyrosinase is:
1) Oxidase 2) Transferase 3) Lyase 4) Isomerase
Ans: 1
77. Mitochondrial DNA is
1) Paternally inherited 2) Maternally inherited 3) Horizontal inheritance 4) Vertical inheritance
Ans: 2
78. Which amino acid migrates fastest on Chromatography on carboxymethyl cellulose medium
1) Aspartic acid 2) Valine 3) Lysin 4) Glycine
Ans: 1
79. Non functional plasma enzymes are all except
1) Alkaline Phosphotase 2) Acid Phophotase 3) Lipoprotein lipase 4) gamma glutamyl transpeptidase
Ans: 3
80. End of chromosomes are replicated by:
1) Telomerase 2) Centromese 3) Restriction Endonuclease 4) Exonuclease
Ans: 1
81. Okazaki segments are required for:
1) DNA synthesis 2) RNA synthesis
3) Protei synthesis 4) None of them
Ans: 1
82. Refsum's disease is due to deficiency of which of the following enzyme
1) Malonate dehydrogenase 2) Thiophorase 3) Succinate thiopase 4) Phytanic acid oxidase
Ans: 4
83. Which process separates the fragments of DNA
1) Gel Centrifugation 2) Paper chromatography
3) High speed centrifugation 4) Thin layer Chromatography
Ans: 1
84. Sulphur containing amino acid is
1) Asparagine 2) Methionine 3) Glycine 4) Alanine
Ans: 2
85. The main function of mitochondria is
1) Protein synthesis 2) Oxidation 3) Electron transfer 4) Fat synthesis
Ans: 2
86. Increased risk of MI is associated with which amino acid
1) Methionine 2) Hymocysteine 3) Ornithine 4) Valine
Ans: 2
87. UV light damage to the DNA leads to
1) Formation of pyrimydine dimers
2) No damage to DNA
3) DNA hydrolysis
4) Double stranded breaks
Ans: 1
88. The tertiary structure of Protein is detected by:
1) X-ray diffraction/Crystallography
2) Spectrophotometry
3) Electrophoresis
4) Chromatography
Ans: 1
89. Vitamin A is stored mainly as Retinolesters in
1) Kidneys 2) Muscle 3) Liver 4) Retina
Ans: 3
90. Enzymes not involved in glycolysis is
1) Enolase
2) Phosphoglyceromutase
3) aldolase
4) Glycerophosphate dehydrogenase
Ans: 4
91. Which of the following has no free aldehyde or ketone group
1) Fructose 2) Maltose 3) Sucrose 4) Galactose
Ans: 3
92. Part of mRNA removed during protein synthesis
1) Intron 2) Codon
3) Exon 4) Cistron
Ans: 1
93. The sugar component of cerebrosides is
1) Fructose 2) Sucrose 3) Galactose 4) Maltose
Ans: 3
94. Mousy odour urine is seen in
1) Maple syrup urine disease 2) PKU 3) Isovaleric aciduria 4) Cystinuria
Ans: 2
95. Pyruvate dehydrogenase contains all except
1) Biotin 2) NAD 3) FAD 4) CoA
Ans: 1
96. Protein are sorted by:
1) Golgi bodies 2) Mitochondria 3) Ribosomes 4) Nuclear Membrane
Ans: 1
97. Muscles are not involved in which glycogen storage disease
1) I
2) II
3) III
4) IV
Ans: 1
98. The activity of carboxylase is dependent upon the positive allosteric effect of:
1) Succinate
2) AMP
3) Isocitrate
4) Acetyl CoA
Ans: 4
99. Ribosomes has following enzymatic activity
1) peptidyl tranferase 2) Peptidase
3) Aminoacycle + RNA synthelase 4) Gtpase
Ans: 1
100. The substance essential for transfer of fatty acids across mitochondrial membrane
1) Creatine 2) Creatinin 3) Carnitine 4) Coenzyme A
Ans: 3